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Dissecting Molecular Phenotypes Through FACS-Based Pooled CRISPR Screens.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2022

Genolet Oriana, Ravid Lustig Liat, Schulz Edda G

Abstract excerpt

Pooled CRISPR screens are emerging as a powerful tool to dissect regulatory networks, by assessing how a protein responds to genetic perturbations in a highly multiplexed manner. A large number of genes are perturbed in a cell population through genomic integration of one single-guide RNA (sgRNA) per cell. A subset of cells with the phenotype of interest can then be enriched through fluorescence-activated cell...

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PMID: 35218528
DOI: 10.1007/7651_2021_457

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