Article
Large-scale image-based profiling of single-cell phenotypes in arrayed CRISPR-Cas9 gene perturbation screens.
Molecular systems biology - 23 Jan 2018
de Groot Reinoud, Lüthi Joel, Lindsay Helen, Holtackers René, Pelkmans Lucas
Abstract excerpt
High-content imaging using automated microscopy and computer vision allows multivariate profiling of single-cell phenotypes. Here, we present methods for the application of the CISPR-Cas9 system in large-scale, image-based, gene perturbation experiments. We show that CRISPR-Cas9-mediated gene perturbation can be achieved in human tissue culture cells in a timeframe that is compatible with image-based phenotyping....
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