Article

CRISPRmap: Sequencing-free optical pooled screens mapping multi-omic phenotypes in cells and tissue

2023-12-26

Abstract excerpt

Pooled genetic screens are powerful tools to study gene function in a high-throughput manner. Typically, sequencing-based screens require cell lysis, which limits the examination of critical phenotypes such as cell morphology, protein subcellular localization, and cell-cell/tissue interactions. In contrast, emerging optical pooled screening methods enable the investigation of these spatial phenotypes in response t...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 1df3e5ef-6fda-53c0-9221-4f89e73efb32
DOI: 10.1101/2023.12.26.572587

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

CRISPRmap: Sequencing-free optical pooled screens mapping multi-omic phenotypes in cells and tissueDOI 10.1101/2023.12.26.572587Read

Mapping multimodal phenotypes to perturbations in cells and tissue with CRISPRmap.Semantic proximity Read

Pooled genetic perturbation screens with image-based phenotypes.Semantic proximity Read

High-throughput, image-based screening of pooled genetic-variant libraries.Semantic proximity Read

FORGE-CRISPR: Reusable Modules for Focused CRISPR Library ConstructionSemantic proximity Read

Optical Pooled Screening for the Discovery of Regulators of the Alternative Lengthening of Telomeres PathwaySemantic proximity Read

STOmics-GenX: CRISPR based approach to improve cell identity specific gene detection from spatially resolved transcriptomicsSemantic proximity Read

CRISPR Functional Genomics in Precision Oncology: Integrating Single-Cell Multi-Omics for Cancer Vulnerability DiscoverySemantic proximity Read

Toward an integrated map of genetic interactions in cancer cells.Semantic proximity Read

High-content imaging-based pooled CRISPR screens in mammalian cells.Semantic proximity Read

Scalable pooled CRISPR screens with single-cell chromatin accessibility profilingSemantic proximity Read

GREPore-seq: A Robust Workflow to Detect Changes after Gene Editing through Long-range PCR and Nanopore SequencingSemantic proximity Read

A Pooled Cell Painting CRISPR Screening Platform Enables de novo Inference of Gene Function by Self-supervised Deep LearningSemantic proximity Read