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Article

Image-based pooled whole-genome CRISPRi screening for subcellular phenotypes.

The Journal of cell biology - 1 Feb 2021

Kanfer Gil, Sarraf Shireen A, Maman Yaakov, Baldwin Heather, Dominguez-Martin Eunice, Johnson Kory R, Ward Michael E, Kampmann Martin, Lippincott-Schwartz Jennifer, Youle Richard J

Abstract excerpt

Genome-wide CRISPR screens have transformed our ability to systematically interrogate human gene function, but are currently limited to a subset of cellular phenotypes. We report a novel pooled screening approach for a wider range of cellular and subtle subcellular phenotypes. Machine learning and convolutional neural network models are trained on the subcellular phenotype to be queried. Genome-wide screening...

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Source

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Identifiers

PMID: 33464298
DOI: 10.1083/jcb.202006180
PMCID: PMC7816647

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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