Article

Cell Type-Agnostic Optical Perturbation Screening Using Nuclear In-Situ Sequencing (NIS-Seq)

2024-01-22

Abstract excerpt

<h4>ABSTRACT</h4> Genome-scale perturbation screening is widely used to identify disease-relevant cellular proteins serving as potential drug targets. However, most biological processes are not compatible with commonly employed perturbation screening methods, which rely on FACS- or growth-based enrichment of cells. Optical pooled screening instead uses fluorescence microscopy to determine the phenotype in single...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 2a613bf0-681f-550f-8487-946df10fa37c
DOI: 10.1101/2024.01.18.576210

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Cell Type-Agnostic Optical Perturbation Screening Using Nuclear In-Situ Sequencing (NIS-Seq)DOI 10.1101/2024.01.18.576210Read

Pooled optical screens in human cellsSemantic proximity Read

Multiome Perturb-seq unlocks scalable discovery of integrated perturbation effects on the transcriptome and epigenomeSemantic proximity Read

A genome-wide atlas of human cell morphologySemantic proximity Read

Pooled CRISPR screening of high-content cellular phenotypes using ghost cytometry.Semantic proximity Read

High-content imaging-based pooled CRISPR screens in mammalian cells.Semantic proximity Read

X-Atlas/Orion: Genome-wide Perturb-seq Datasets via a Scalable Fix-Cryopreserve Platform for Training Dose-Dependent Biological Foundation ModelsSemantic proximity Read

Linking live-cell behavior to transcriptional responses across perturbations using dynamic cagingSemantic proximity Read

Pooled genetic perturbation screens with image-based phenotypes.Semantic proximity Read

Morphological map of under- and over-expression of genes in human cellsSemantic proximity Read

EnrichSci: Transcript-guided Targeted Cell Enrichment for Scalable Single-Cell RNA SequencingSemantic proximity Read

txci-ATAC-seq, a massive-scale single-cell technique to profile chromatin accessibilitySemantic proximity Read

Mapping multimodal phenotypes to perturbations in cells and tissue with CRISPRmap.Semantic proximity Read