Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypes.

eLife - 8 Jan 2021

Kroll François, Powell Gareth T, Ghosh Marcus, Gestri Gaia, Antinucci Paride, Hearn Timothy J, Tunbak Hande, Lim Sumi, Dennis Harvey W, Fernandez Joseph M, Whitmore David, Dreosti Elena, Wilson Stephen W, Hoffman Ellen J, Rihel Jason

Abstract excerpt

Hundreds of human genes are associated with neurological diseases, but translation into tractable biological mechanisms is lagging. Larval zebrafish are an attractive model to investigate genetic contributions to neurological diseases. However, current CRISPR-Cas9 methods are difficult to apply to large genetic screens studying behavioural phenotypes. To facilitate rapid genetic screening, we developed a simple...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Behavior, Animal
Share to CRISPR-Cas Systems

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Embryo, Nonmammalian

Share to Gene Knockout Techniques

Share to Genetic Testing

Share to Phenotype

Share to RNA, Guide, CRISPR-Cas Systems

Share to Zebrafish

Source

Open in PubMed Open full text

Identifiers

PMID: 33416493
DOI: 10.7554/eLife.59683
PMCID: PMC7793621

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypes.PMID 33416493Read ↗

A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypesSemantic proximity Read ↗

Optimizing gRNA selection for high-penetrance F0 CRISPR screening for interrogating disease gene function.Semantic proximity Read ↗

CRISPR/Cas9-Based Mutagenesis Strategies for Efficient Biallelic Gene Inactivation and Consistent Phenotypic Detection in F0 Zebrafish.Semantic proximity Read ↗

A Zebrafish-Based Platform for High-Throughput Epilepsy Modeling and Drug Screening in F0.Semantic proximity Read ↗

Zebrafish models of candidate human epilepsy-associated genes provide evidence of hyperexcitabilitySemantic proximity Read ↗

Orphan cytochrome P450 20A1 CRISPR/Cas 9 mutants and neurobehavioral phenotypes in zebrafishSemantic proximity Read ↗

Mutagenesis and phenotyping resources in zebrafish for studying development and human disease.Semantic proximity Read ↗

CRIMP: a CRISPR/Cas9 insertional mutagenesis protocol and toolkit.Semantic proximity Read ↗

Targeted candidate gene screens using CRISPR/Cas9 technology.Semantic proximity Read ↗

Acute multi-sgRNA knockdown of KEOPS complex genes reproduces the microcephaly phenotype of the stable knockout zebrafish model.Semantic proximity Read ↗

New Developments in CRISPR/Cas-based Functional Genomics and their Implications for Research Using Zebrafish.Semantic proximity Read ↗

Evaluation of CRISPR gene-editing tools in zebrafishSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.