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Article

Optimizing gRNA selection for high-penetrance F0 CRISPR screening for interrogating disease gene function.

Nucleic acids research - 27 Feb 2025

Lin Sheng-Jia, Huang Kevin, Petree Cassidy, Qin Wei, Varshney Pratishtha, Varshney Gaurav K

Abstract excerpt

Genes and genetic variants associated with human disease are continually being discovered, but validating their causative roles and mechanisms remains a significant challenge. CRISPR/Cas9 genome editing in model organisms like zebrafish can enable phenotypic characterization of founder generation (F0) knockouts (Crispants), but existing approaches are not amenable to high-throughput genetic screening due to high...

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Identifiers

PMID: 40103232
DOI: 10.1093/nar/gkaf180
PMCID: PMC11915512

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