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Article

SpRY greatly expands the genome editing scope in rice with highly flexible PAM recognition.

Genome biology - 4 Jan 2021

Xu Ziyan, Kuang Yongjie, Ren Bin, Yan Daqi, Yan Fang, Spetz Carl, Sun Wenxian, Wang Guirong, Zhou Xueping, Zhou Huanbin

Abstract excerpt

BACKGROUND: Plant genome engineering mediated by various CRISPR-based tools requires specific protospacer adjacent motifs (PAMs), such as the well-performed NGG, NG, and NNG, to initiate target recognition, which notably restricts the editable range of the plant genome. RESULTS: In this study, we thoroughly investigate the nuclease activity and the PAM preference of two structurally engineered SpCas9 variants,...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 33397431
DOI: 10.1186/s13059-020-02231-9
PMCID: PMC7780387

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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