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Rapid and Continuous Directed Evolution in Vibrio natriegens Utilizing an In Vivo Hypermutation System.

ACS synthetic biology - 17 Jul 2026

Zhu Ruiying, Bao Zehua

Abstract excerpt

Genetic diversification serves as the foundation for in vivo molecular evolution. The fusion of a cytidine deaminase with T7 RNA polymerase (MutaT7) enables efficient diversification of DNA sequences downstream of the T7 promoter in model microbial hosts E. coli and S. cerevisiae. Vibrio natriegens, currently the fastest-growing nonpathogenic bacterium with a doubling time half that of E. coli, emerged as a...

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PMID: 42362483
DOI: 10.1021/acssynbio.5c00936

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026.

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