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MutaT7GDE: A Single Chimera for the Targeted, Balanced, Efficient, and Processive Installation of All Possible Transition Mutations In Vivo.

ACS synthetic biology - 20 Sept 2024

Mengiste Amanuella A, McDonald Julie L, Nguyen Tran Minh Thuan, Plank Anna V, Wilson Robert H, Butty Vincent L, Shoulders Matthew D

Abstract excerpt

Deaminase-T7 RNA polymerase fusion (MutaT7) proteins are a growing class of synthetic biology tools used to diversify target genes during in vivo laboratory evolution. To date, MutaT7 chimeras comprise either a deoxyadenosine or deoxycytidine deaminase fused to a T7 RNA polymerase. Their expression drives targeted deoxyadenosine-to-deoxyguanosine or deoxycytidine-to-deoxythymidine mutagenesis, respectively. Here,...

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PMID: 39190860
DOI: 10.1021/acssynbio.4c00316

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