Article

A dual gene-specific mutator system installs all transition mutations at similar rates <i>in vivo</i>

2022-06-11

Abstract excerpt

<h4>ABSTRACT</h4> Targeted in vivo hypermutation accelerates directed evolution of proteins through concurrent DNA diversification and selection. Among recently developed methods, the systems employing a fusion protein of a nucleobase deaminase and T7 RNA polymerase present gene-specific targeting. However, their mutational spectra have been largely limited to exclusive or dominant C:G→T:A mutations. Here we des...

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Literature Corpus work: c95da87f-656d-5726-85ce-64e79e97c8a0
DOI: 10.1101/2022.06.09.495438

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