A dual gene-specific mutator system installs all transition mutations at similar frequencies in vivo.

Abstract excerpt

Targeted in vivo hypermutation accelerates directed evolution of proteins through concurrent DNA diversification and selection. Although systems employing a fusion protein of a nucleobase deaminase and T7 RNA polymerase present gene-specific targeting, their mutational spectra have been limited to exclusive or dominant C:G→T:A mutations. Here we describe eMutaT7transition, a new gene-specific hypermutation...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Gene Editing
• Share to Mutation
• Share to Mutation Rate