Article
Determination of low-abundance single-base point mutations based on endonuclease IV and branch migration system.
Analytica chimica acta - 16 Oct 2020
Tang Xiaofeng, Chen Na, Liu Ruijie, Hu Qingyi, Liu Na, Xiao Xianjin
Abstract excerpt
Combining endonuclease IV and branch migration competition systems, we have designed a new single-base mutation detection method which is capable of thermostatic, sensitive, simple, and cost-effective at the same time, while other probe method based on endonuclease IV hardly achieve. Our method has better discrimination factors (6.81-83.10) and a low abundance detection limit of mutant-type DNA (MT) (0.05%...
Topics
- Deoxyribonuclease IV (Phage T4-Induced)
- Mutation
- Point Mutation