Article
A novel method to quantify base substitution mutations at the 10-6 per bp level in DNA samples.
Cancer letters - 10 Sept 2017
Yamashita Satoshi, Iida Naoko, Takeshima Hideyuki, Hattori Naoko, Maeda Masahiro, Kishino Takayoshi, Nagano Reiko, Shimazu Taichi, Tsugane Shoichiro, Ushijima Toshikazu
Abstract excerpt
Somatic base substitution mutations of frequencies at the 10-6/bp level are expected to be present in many biomedical samples, such as tissues exposed to carcinogenic factors and exhausted stem cells. However, measurement of such rare mutations has been very difficult in human DNA samples. Here, we invented the use of 100 copies of genomic DNA as a template for amplicon deep sequencing so that a real mutation in...
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