Simultaneous genotyping of multiple somatic mutations by using a clamping PNA and PNA detection probes.

Abstract excerpt

It has been very difficult to detect and quantify multiple somatic mutations simultaneously in single-tube qPCR. Here, a novel method for simultaneous detection of multiple mutations and a melting curve analysis was developed by using clamping PNA and detection PNA probes. Each PNA probe was designed to have a specific melting temperature by the introduction of γ-PNA monomer. This technique was successfully...

Topics

• Alanine
• Cell Line
• Dimerization
• Genes, ras
• Genotyping Techniques
• HeLa Cells
• Humans
• Lysine
• Molecular Probes
• Mutation
• Peptide Nucleic Acids