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Article

Simultaneous genotyping of multiple somatic mutations by using a clamping PNA and PNA detection probes.

Chembiochem : a European journal of chemical biology - 19 Jan 2015

Kim Yong-Tae, Kim Jin Woo, Kim Sung Kee, Joe Goon Ho, Hong In Seok

Abstract excerpt

It has been very difficult to detect and quantify multiple somatic mutations simultaneously in single-tube qPCR. Here, a novel method for simultaneous detection of multiple mutations and a melting curve analysis was developed by using clamping PNA and detection PNA probes. Each PNA probe was designed to have a specific melting temperature by the introduction of γ-PNA monomer. This technique was successfully...

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PMID: 25534284
DOI: 10.1002/cbic.201402640

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