Article
Site-specific insertion of endonuclease recognition sites into amplicons to improve post-PCR analysis sensitivity of gene mutation.
Biosensors & bioelectronics - 15 Jul 2022
Song Lin, Li Junjie, Chen Kena, Zuo Chen, Wu You, Bai Dan, Zhao Lin, Yang Yujun, Liu Chenggui, Xie Guoming
Abstract excerpt
Precise detection of low-frequency gene mutations surrounded by excess wild-type DNA is important in many aspects of medical fields. Most hybridization-based methods for high-resolution mutant allele analysis are hindered by competition of the complementary strand with single-strand probes for the target strand. Here, we demonstrate that site-specific insertion of endonuclease recognition sites into amplicons...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.