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Guiding-Strand-Controlled DNA Nucleases with Enhanced Specificity and Tunable Kinetics for DNA Mutation Detection.

Analytical chemistry - 11 May 2021

Ming Zhihao, Zhang Wei, Lin Meng, Tang Xiaofeng, Chen Na, Liu Na, Xin Xiaoyan, Wang Hongbo, Xiang Wenpei, Xiao Xianjin

Abstract excerpt

Nucleases are powerful tools in various biomedical applications, such as genetic engineering, biosensing, and molecular diagnosis. However, the commonly used nucleases (endonuclease IV, apurinic/apyrimidinic endonuclease-1, and λ exonuclease) are prone to the nonspecific cleavage of single-stranded DNA, making the desired reactions extremely low-yield and unpredictable. Herein, we have developed...

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Identifiers

PMID: 33900739
DOI: 10.1021/acs.analchem.1c00507

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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