Article
Long-read sequencing for non-small-cell lung cancer genomes.
Genome research - 1 Sept 2020
Sakamoto Yoshitaka, Xu Liu, Seki Masahide, Yokoyama Toshiyuki T, Kasahara Masahiro, Kashima Yukie, Ohashi Akihiro, Shimada Yoko, Motoi Noriko, Tsuchihara Katsuya, Kobayashi Susumu S, Kohno Takashi, Shiraishi Yuichi, Suzuki Ayako, Suzuki Yutaka
Abstract excerpt
Here, we report the application of a long-read sequencer, PromethION, for analyzing human cancer genomes. We first conducted whole-genome sequencing on lung cancer cell lines. We found that it is possible to genotype known cancerous mutations, such as point mutations. We also found that long-read sequencing is particularly useful for precisely identifying and characterizing structural aberrations, such as large...
Topics
- Adenocarcinoma
- Carcinoma, Non-Small-Cell Lung
- Cell Line, Tumor
- Chromosome Aberrations
- DNA Copy Number Variations
- Female
- Gene Expression Profiling
- Gene Rearrangement
- Genes, Neoplasm