The combinational use of <scp>CRISPR</scp>/Cas9‐based gene editing and targeted toxin technology enables efficient biallelic knockout of the α‐1,3‐galactosyltransferase gene in porcine embryonic fibroblasts

Abstract excerpt

BACKGROUND: The recent development of the type II clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system has enabled genome editing of mammalian genomes including those of mice and human; however, its applicability and efficiency in the pig have not been studied in depth. Here, using the CRISPR/Cas9 system, we aimed to destroy the function of the porcine α-1,3-galactosyltransferase...

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