Article
GOTI, a method to identify genome-wide off-target effects of genome editing in mouse embryos.
Nature protocols - 1 Sept 2020
Zuo Erwei, Sun Yidi, Wei Wu, Yuan Tanglong, Ying Wenqin, Sun Hao, Yuan Liyun, Steinmetz Lars M, Li Yixue, Yang Hui
Abstract excerpt
Genome editing holds great potential for correcting pathogenic mutations. We developed a method called GOTI (genome-wide off-target analysis by two-cell embryo injection) to detect off-target mutations by editing one blastomere of two-cell mouse embryos using either CRISPR-Cas9 or base editors. GOTI directly compares edited and non-edited cells without the interference of genetic background and thus could detect...
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