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Article

Sortase mutants with improved protein thermostability and enzymatic activity obtained by consensus design.

Protein engineering, design & selection : PEDS - 31 Dec 2019

Wójcik Magdalena, Vázquez Torres Susana, Quax Wim J, Boersma Ykelien L

Abstract excerpt

Staphylococcus aureus sortase A (SaSrtA) is an enzyme that anchors proteins to the cell surface of Gram-positive bacteria. During the transpeptidation reaction performed by SaSrtA, proteins containing an N-terminal glycine can be covalently linked to another protein with a C-terminal LPXTG motif (X being any amino acid). Since the sortase reaction can be performed in vitro as well, it has found many applications...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 32725168
DOI: 10.1093/protein/gzaa018

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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