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Article

Sequence variation in the β7-β8 loop of bacterial class A sortase enzymes alters substrate selectivity.

The Journal of biological chemistry - 1 Aug 2021

Piper Isabel M, Struyvenberg Sarah A, Valgardson Jordan D, Johnson D Alex, Gao Melody, Johnston Katherine, Svendsen Justin E, Kodama Hanna M, Hvorecny Kelli L, Antos John M, Amacher Jeanine F

Abstract excerpt

Gram-positive bacteria contain sortase enzymes on their cell surfaces that catalyze transpeptidation reactions critical for proper cellular function. In vitro, sortases are used in sortase-mediated ligation (SML) reactions for a variety of protein engineering applications. Historically, sortase A from Staphylococcus aureus (saSrtA) has been the enzyme of choice to catalyze SML reactions. However, the stringent...

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Identifiers

PMID: 34302812
DOI: 10.1016/j.jbc.2021.100981
PMCID: PMC8361268

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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