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Article

Engineering sortase A by screening a second-generation library using phage display.

Journal of peptide science : an official publication of the European Peptide Society - 1 Jul 2017

Schmohl Lena, Bierlmeier Jan, Gerth Fabian, Freund Christian, Schwarzer Dirk

Abstract excerpt

Sortase-mediated ligation is one of the most commonly used chemo-enzymatic techniques for the site-specific modification of proteins. We have established a new library of sortase mutants for directed evolution of sortase substrate selectivity. Phage display screens of this second-generation library yielded sortase mutants that ligate substrate proteins containing an APxTG or FPxTG recognition sequence instead of...

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PMID: 28185387
DOI: 10.1002/psc.2980

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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