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Article

Generation of Ca2+-independent sortase A mutants with enhanced activity for protein and cell surface labeling.

PloS one - 1 Jan 2017

Jeong Hee-Jin, Abhiraman Gita C, Story Craig M, Ingram Jessica R, Dougan Stephanie K

Abstract excerpt

Sortase A, a calcium-dependent transpeptidase derived from Staphylococcus aureus, is used in a broad range of applications, such as the conjugation of fluorescent dyes and other moieties to proteins or to the surface of eukaryotic cells. In vivo and cell-based applications of sortase have been somewhat limited by the large range of calcium concentrations, as well as by the often transient nature of...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 29200433
DOI: 10.1371/journal.pone.0189068
PMCID: PMC5714338

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