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Article

Site-specific protein modification using immobilized sortase in batch and continuous-flow systems.

Nature protocols - 1 Mar 2015

Witte Martin D, Wu Tongfei, Guimaraes Carla P, Theile Christopher S, Blom Annet E M, Ingram Jessica R, Li Zeyang, Kundrat Lenka, Goldberg Shalom D, Ploegh Hidde L

Abstract excerpt

Transpeptidation catalyzed by sortase A allows the preparation of proteins that are site-specifically and homogeneously modified with a wide variety of functional groups, such as fluorophores, PEG moieties, lipids, glycans, bio-orthogonal reactive groups and affinity handles. This protocol describes immobilization of sortase A on a solid support (Sepharose beads). Immobilization of sortase A simplifies downstream...

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Identifiers

PMID: 25719269
DOI: 10.1038/nprot.2015.026
PMCID: PMC4757899

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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