Article
CRISPR/Cas9 as a Mutagenic Factor.
International journal of molecular sciences - 9 Jan 2024
Shumega Andrey R, Pavlov Youri I, Chirinskaite Angelina V, Rubel Aleksandr A, Inge-Vechtomov Sergey G, Stepchenkova Elena I
Abstract excerpt
The discovery of the CRISPR/Cas9 microbial adaptive immune system has revolutionized the field of genetics, by greatly enhancing the capacity for genome editing. CRISPR/Cas9-based editing starts with DNA breaks (or other lesions) predominantly at target sites and, unfortunately, at off-target genome sites. DNA repair systems differing in accuracy participate in establishing desired genetic changes but also...
Topics
- Mutagens
- CRISPR-Cas Systems
- RNA, Guide, CRISPR-Cas Systems
- Mutagenesis
- Mutation