Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Perfectly matched 20-nucleotide guide RNA sequences enable robust genome editing using high-fidelity SpCas9 nucleases.

Genome biology - 11 Oct 2017

Zhang Dingbo, Zhang Huawei, Li Tingdong, Chen Kunling, Qiu Jin-Long, Gao Caixia

Abstract excerpt

High-fidelity SpCas9 variants (eSpCas9 and SpCas9-HF1) have been engineered to reduce off-target effects. We found that changes in guide RNA length induced significant reductions in the editing activities of SpCas9 variants in plant cells. Single guide RNAs harboring precise, perfectly matched 20-nucleotide guide sequences are necessary for high on-target editing activities of eSpCas9 and SpCas9-HF1. Precise...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Bacterial Proteins
Share to Base Sequence

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to CRISPR-Associated Protein 9

Share to Endonucleases

Share to Gene Editing

Share to Protein Engineering

Share to Substrate Specificity

Source

Open in PubMed Open full text

Identifiers

PMID: 29020979
DOI: 10.1186/s13059-017-1325-9
PMCID: PMC5637269

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Perfectly matched 20-nucleotide guide RNA sequences enable robust genome editing using high-fidelity SpCas9 nucleases.PMID 29020979Read ↗

Blackjack mutations improve the on-target activities of increased fidelity variants of SpCas9 with 5'G-extended sgRNAs.Semantic proximity Read ↗

Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage.Semantic proximity Read ↗

Shortening the sgRNA-DNA interface enables SpCas9 and eSpCas9(1.1) to nick the target DNA strand.Semantic proximity Read ↗

Selection of highly efficient sgRNAs for CRISPR/Cas9-based plant genome editing.Semantic proximity Read ↗

Boosting genome editing efficiency in human cells and plants with novel LbCas12a variantsSemantic proximity Read ↗

An engineered Cas12i nuclease that is an efficient genome editing tool in animals and plantsSemantic proximity Read ↗

Programmable multi-kilobase RNA editing using CRISPR-mediated trans-splicingSemantic proximity Read ↗

Combination of engineered FnCas9 and extended gRNAs for PAM-flexible, robust and nucleobase specific editing and diagnosticsSemantic proximity Read ↗

Pooled CRISPR screening at high sensitivity with an empirically designed sgRNA librarySemantic proximity Read ↗

Genome editing mediated by SpCas9 variants with broad non-canonical PAM compatibility in plants.Semantic proximity Read ↗

Introduction of mutations in plants with prime editing.Semantic proximity Read ↗

Expanding base editing scope to near-PAMless with engineered CRISPR/Cas9 variants in plantsSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.