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Article

A lentivirus-free inducible CRISPR-Cas9 system for efficient targeting of human genes.

Analytical biochemistry - 1 Aug 2017

Bisht Kamlesh, Grill Sherilyn, Graniel Jacqueline, Nandakumar Jayakrishnan

Abstract excerpt

CRISPR-Cas9 is a cutting-edge tool for modifying genomes. The efficacy with which Cas9 recognizes its target has revolutionized the engineering of knockouts. However this efficacy complicates the knocking out of important genes in cultured cells. Unedited cells holding a survival advantage within an edited population can confound the knockout phenotype. Here we develop a HeLa-based system that overcomes this...

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Identifiers

PMID: 28477963
DOI: 10.1016/j.ab.2017.05.001
PMCID: PMC5501077

Genes

AGO2

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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