Article
A protocol for custom CRISPR Cas9 donor vector construction to truncate genes in mammalian cells using pcDNA3 backbone.
BMC molecular biology - 14 Mar 2018
Vazquez Neftali, Sanchez Lilia, Marks Rebecca, Martinez Eduardo, Fanniel Victor, Lopez Alma, Salinas Andrea, Flores Itzel, Hirschmann Jesse, Gilkerson Robert, Schuenzel Erin, Dearth Robert, Halaby Reginald, Innis-Whitehouse Wendy, Keniry Megan
Abstract excerpt
BACKGROUND: Clustered regularly interspaced short palindromic repeat (CRISPR) RNA-guided adaptive immune systems are found in prokaryotes to defend cells from foreign DNA. CRISPR Cas9 systems have been modified and employed as genome editing tools in wide ranging organisms. Here, we provide a det...
Topics
- CRISPR-Cas Systems
- Cell Line
- Deoxyribonuclease I
- Forkhead Box Protein O3
- Gene Editing
- Genetic Vectors
- HEK293 Cells
- Homologous Recombination
- Humans
- Male
- Mutation
- Plasmids
- RNA, Guide, CRISPR-Cas Systems