Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

A protocol for custom CRISPR Cas9 donor vector construction to truncate genes in mammalian cells using pcDNA3 backbone.

BMC molecular biology - 14 Mar 2018

Vazquez Neftali, Sanchez Lilia, Marks Rebecca, Martinez Eduardo, Fanniel Victor, Lopez Alma, Salinas Andrea, Flores Itzel, Hirschmann Jesse, Gilkerson Robert, Schuenzel Erin, Dearth Robert, Halaby Reginald, Innis-Whitehouse Wendy, Keniry Megan

Abstract excerpt

BACKGROUND: Clustered regularly interspaced short palindromic repeat (CRISPR) RNA-guided adaptive immune systems are found in prokaryotes to defend cells from foreign DNA. CRISPR Cas9 systems have been modified and employed as genome editing tools in wide ranging organisms. Here, we provide a detailed protocol to truncate genes in mammalian cells using CRISPR Cas9 editing. We describe custom donor vector...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Gene Editing
Share to Cell Line

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to CRISPR-Cas Systems

Share to Deoxyribonuclease I

Share to Genetic Vectors

Share to HEK293 Cells

Share to Mutation

Share to Plasmids

Source

Open in PubMed Open full text

Identifiers

PMID: 29540148
DOI: 10.1186/s12867-018-0105-8
PMCID: PMC5853148

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

A protocol for custom CRISPR Cas9 donor vector construction to truncate genes in mammalian cells using pcDNA3 backbone.PMID 29540148Read ↗

CRISPR-Cas9 Genome Editing in Human Cell Lines with Donor Vector Made by Gibson Assembly.Semantic proximity Read ↗

CRISPR-Cas12a-assisted PCR tagging of mammalian genes.Semantic proximity Read ↗

Establishment of the CRISPR/Cas9 System for Targeted Gene Disruption and Gene Tagging.Semantic proximity Read ↗

A strategy for genome-wide seamless tagging of human protein-coding genesSemantic proximity Read ↗

Self-cutting and integrating CRISPR plasmids (SCIPs) enable targeted genomic integration of genetic payloads for rapid cell engineeringSemantic proximity Read ↗

Homologous Recombination-Independent Large Gene Cassette Knock-in in CHO Cells Using TALEN and MMEJ-Directed Donor PlasmidsSemantic proximity Read ↗

Rapid, modular, and cost-effective generation of donor DNA constructs for CRISPR-based gene knock-inSemantic proximity Read ↗

Rapid generation of homozygous fluorescent knock-in human cells using CRISPR/Cas9 genome editing and validation by automated imaging and digital PCR screeningSemantic proximity Read ↗

CRISPR‐interceded CHO cell line development approachesSemantic proximity Read ↗

The method of choice to knock-in large inserts via CRISPRSemantic proximity Read ↗

GeneWeld: a method for efficient targeted integration directed by short homologySemantic proximity Read ↗

CRISPR-based shuttle cloning of 1,397 human genes into UAS vectorsSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.