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Article

CRISPR Co-Editing Strategy for Scarless Homology-Directed Genome Editing.

International journal of molecular sciences - 3 Apr 2021

Reuven Nina, Adler Julia, Myers Nadav, Shaul Yosef

Abstract excerpt

The clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 has revolutionized genome editing by providing a simple and robust means to cleave specific genomic sequences. However, introducing templated changes at the targeted site usually requires homology-directed repair (HDR), active in only a small subset of cells in culture. To enrich for HDR-dependent edited cells, we employed a co-editing...

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Identifiers

PMID: 33916763
DOI: 10.3390/ijms22073741
PMCID: PMC8038335

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Source receipt: pubmed, retrieved 7 Sept 2026.

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