Article

Self-cutting and integrating CRISPR plasmids (SCIPs) enable targeted genomic integration of genetic payloads for rapid cell engineering

2020-03-25

Abstract excerpt

Since observations that CRISPR nucleases function in mammalian cells, many strategies have been devised to adapt them for genetic engineering. Here, we investigated self-cutting and integrating CRISPR-Cas9 plasmids (SCIPs) as easy-to-use gene editing tools that insert themselves at CRISPR-guided locations. SCIPs demonstrated similar expression kinetics and gene disruption efficiency in mouse (EL4) and human (Jurka...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: a3a0aff4-af25-597b-b21d-9755555a92ea
DOI: 10.1101/2020.03.25.008276

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Self-cutting and integrating CRISPR plasmids (SCIPs) enable targeted genomic integration of genetic payloads for rapid cell engineeringDOI 10.1101/2020.03.25.008276Read

High-efficiency nonviral CRISPR/Cas9-mediated gene editing of human T cells using plasmid donor DNASemantic proximity Read

CRISPR/Cas-based CAR-T cells: production and applicationSemantic proximity Read

The CRISPR-Cas12a Platform for Accurate Genome Editing, Gene Disruption, and Efficient Transgene Integration in Human Immune CellsSemantic proximity Read

CRISPR-Mediated Non-Viral Site-Specific Gene Integration and Expression in T Cells: Protocol and Application for T-Cell TherapySemantic proximity Read

CRISPR-Cas12a-assisted PCR tagging of mammalian genes.Semantic proximity Read

Applications of CRISPR Genome Editing to Advance the Next Generation of Adoptive Cell Therapies for CancerSemantic proximity Read

Boosting CAR-T cell therapy with CRISPR technologySemantic proximity Read

Establishment of the CRISPR/Cas9 System for Targeted Gene Disruption and Gene Tagging.Semantic proximity Read

Efficient nonviral integration of large transgenes into human T cells using Cas9-CLIPTSemantic proximity Read

Leveraging CRISPR gene editing technology to optimize the efficacy, safety and accessibility of CAR T-cell therapySemantic proximity Read

The transformative potential of AI-driven CRISPR-Cas9 genome editing to enhance CAR T-cell therapySemantic proximity Read

Hybrid ssDNA repair templates enable high yield genome engineering in primary cells for disease modeling and cell therapy manufacturingSemantic proximity Read