Article
Analysis of unusual and signature APOBEC-mutations in HIV-1 pol next-generation sequences.
PloS one - 1 Jan 2020
Tzou Philip L, Kosakovsky Pond Sergei L, Avila-Rios Santiago, Holmes Susan P, Kantor Rami, Shafer Robert W
Abstract excerpt
INTRODUCTION: At low mutation-detection thresholds, next generation sequencing (NGS) for HIV-1 genotypic resistance testing is susceptible to artifactual detection of mutations arising from PCR error and APOBEC-mediated G-to-A hypermutation. METHODS: We analyzed published HIV-1 pol Illumina NGS data to characterize the distribution of mutations at eight NGS mutation detection thresholds: 20%, 10%, 5%, 2%, 1%,...
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