Multi-Laboratory Comparison of Next-Generation to Sanger-Based Sequencing for HIV-1 Drug Resistance Genotyping.

doi:10.3390/v12070694

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Article

Multi-Laboratory Comparison of Next-Generation to Sanger-Based Sequencing for HIV-1 Drug Resistance Genotyping.

Viruses - 27 Jun 2020

Parkin Neil T, Avila-Rios Santiago, Bibby David F, Brumme Chanson J, Eshleman Susan H, Harrigan P Richard, Howison Mark, Hunt Gillian, Ji Hezhao, Kantor Rami, Ledwaba Johanna, Lee Emma R, Matías-Florentino Margarita, Mbisa Jean L, Noguera-Julian Marc, Paredes Roger, Rivera-Amill Vanessa, Swanstrom Ronald, Zaccaro Daniel J, Zhang Yinfeng, Zhou Shuntai, Jennings Cheryl

Abstract excerpt

Next-generation sequencing (NGS) is increasingly used for HIV-1 drug resistance genotyping. NGS methods have the potential for a more sensitive detection of low-abundance variants (LAV) compared to standard Sanger sequencing (SS) methods. A standardized threshold for reporting LAV that generates data comparable to those derived from SS is needed to allow for the comparability of data from laboratories using NGS...

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PMID: 32605062
DOI: 10.3390/v12070694
PMCID: PMC7411816

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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