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Article

Chemoenzymatic Semisynthesis of Phosphorylated α-Synuclein Enables Identification of a Bidirectional Effect on Fibril Formation.

ACS chemical biology - 20 Mar 2020

Pan Buyan, Rhoades Elizabeth, Petersson E James

Abstract excerpt

Post-translational modifications (PTMs) impact the pathological aggregation of α-synuclein (αS), a hallmark of Parkinson's disease (PD). Here, we synthesize αS phosphorylated at tyrosine 39 (pY39) through a novel route using in vitro enzymatic phosphorylation of a fragment followed by ligation to form the full-length protein. We can execute this synthesis in combination with unnatural amino acid mutagenesis to...

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Identifiers

PMID: 32065743
DOI: 10.1021/acschembio.9b01038
PMCID: PMC7724256

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