Article
Modulated control of DNA supercoiling balance by the DNA-wrapping domain of bacterial gyrase.
Nucleic acids research - 28 Feb 2020
Hobson Matthew J, Bryant Zev, Berger James M
Abstract excerpt
Negative supercoiling by DNA gyrase is essential for maintaining chromosomal compaction, transcriptional programming, and genetic integrity in bacteria. Questions remain as to how gyrases from different species have evolved profound differences in their kinetics, efficiency, and extent of negative supercoiling. To explore this issue, we analyzed homology-directed mutations in the C-terminal, DNA-wrapping domain...
Topics
- Adenosine Triphosphate
- Catalysis
- Chromosomes, Bacterial
- DNA Gyrase
- DNA, Bacterial
- DNA, Superhelical
- Escherichia coli
- Models, Molecular
- Mutation
- Nucleic Acid Conformation
- Protein Binding