Article
Improvement of the Thermostability and Activity of Pullulanase from Anoxybacillus sp. WB42.
Applied biochemistry and biotechnology - 1 Jul 2020
Pang Bo, Zhou Li, Cui Wenjing, Liu Zhongmei, Zhou Zhemin
Abstract excerpt
Pullulanase is a commonly used starch-debranching enzyme with broad application in food, chemical and pharmaceutical industries. Since the starch-debranching process requires a high temperature, a thermostable pullulanase is desirable. In this study, based on the strategy of surficial residue replacement and disulfide bond introduction, a mutant pullulanase (PulAC) derived from the pullulanase (PulA) of...
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