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Article

Development of pullulanase mutants to enhance starch substrate utilization for efficient production of β-CD.

International journal of biological macromolecules - 31 Jan 2021

Li Xiaoxiao, Ji Hangyan, Bai Yuxiang, Jin Zhengyu

Abstract excerpt

The inhibitory effect of β-CD on pullulanase which hydrolyzes α-1,6 glycosidic bond in starch to release more available linear substrates, limited substrate utilization thus influencing the yield of β-CD. Here, an aspartic acid residue (D465) which interacted with cyclodextrin ligand by hydrogen bond, was mutated to explore its contribution to bind inhibitors and obtain mutants with lower affinity to β-CD. Enzyme...

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Identifiers

PMID: 33220368
DOI: 10.1016/j.ijbiomac.2020.11.120

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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