Article
Disorder prediction-based construct optimization improves activity and catalytic efficiency of Bacillus naganoensis pullulanase.
Scientific reports - 19 Apr 2016
Wang Xinye, Nie Yao, Mu Xiaoqing, Xu Yan, Xiao Rong
Abstract excerpt
Pullulanase is a well-known starch-debranching enzyme. However, the production level of pullulanase is yet low in both wide-type strains and heterologous expression systems. We predicted the disorder propensities of Bacillus naganoensis pullulanase (PUL) using the bioinformatics tool, Disorder Prediction Meta-Server. On the basis of disorder prediction, eight constructs, including PULΔN5, PULΔN22, PULΔN45,...
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