Phenylalanine476 mutation of pullulanase from Bacillus subtilis str. 168 improves the starch substrate utilization by weakening the product β-cyclodextrin inhibition.

Abstract excerpt

Since β-cyclodextrin (β-CD) was the competitive inhibitor of pullulanase, substrate utilization and product inhibition were a conflict in β-CD production when using pullulanase for debranching to release more convertible substrate. Here, a phenylalanine complexed with cyclodextrin ligand via classic hydrophobic interaction and hydrogen bond in the crystal structure of pullulanase and absolutely conserved in all...

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