Article
Structure-based engineering of methionine residues in the catalytic cores of alkaline amylase from Alkalimonas amylolytica for improved oxidative stability.
Applied and environmental microbiology - 1 Nov 2012
Yang Haiquan, Liu Long, Wang Mingxing, Li Jianghua, Wang Nam Sun, Du Guocheng, Chen Jian
Abstract excerpt
This work aims to improve the oxidative stability of alkaline amylase from Alkalimonas amylolytica through structure-based site-directed mutagenesis. Based on an analysis of the tertiary structure, five methionines (Met 145, Met 214, Met 229, Met 247, and Met 317) were selected as the mutation sites and individually replaced with leucine. In the presence of 500 mM H(2)O(2) at 35°C for 5 h, the wild-type enzyme...
Topics
- Amylases
- Biocatalysis
- Catalytic Domain
- Detergents
- Gammaproteobacteria
- Hot Temperature
- Methionine
- Molecular Sequence Data
- Mutation
- Oxidation-Reduction
- Oxidative Stress
- Protein Structure, Tertiary
- Surface-Active Agents