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Article

Improving the Thermostability of Acidic Pullulanase from Bacillus naganoensis by Rational Design.

PloS one - 1 Jan 2016

Chang Meihui, Chu Xiaoyu, Lv Jinzhi, Li Qingbin, Tian Jian, Wu Ningfeng

Abstract excerpt

Pullulanase (EC 3.2.1.41) plays an important role in the specific hydrolysis of branch points in amylopectin. Enhancing its thermostability is required for its industrial application. In this study, rational protein design was used to improve the thermostability of PulB from Bacillus naganoensis (AB231790.1), which has strong enzymatic properties. Three positive single-site mutants (PulB-D328H, PulB-N387D, and...

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Identifiers

PMID: 27764201
DOI: 10.1371/journal.pone.0165006
PMCID: PMC5072709

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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