Article
Performance evaluation of the Vela Dx Sentosa next-generation sequencing system for HIV-1 DNA genotypic resistance.
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology - 1 Jan 2020
Raymond Stéphanie, Nicot Florence, Abravanel Florence, Minier Luce, Carcenac Romain, Lefebvre Caroline, Harter Agnès, Martin-Blondel Guillaume, Delobel Pierre, Izopet Jacques
Abstract excerpt
BACKGROUND: Patients on antiretroviral therapy could benefit from HIV-1 DNA resistance genotyping for exploring virological failure with low viral load or to guide treatment simplification. Few new generation sequencing data are available. OBJECTIVE: To check that the automated deep sequencing Sentosa platform (Vela DX) detected minority resistant variants well enough for HIV DNA genotyping. STUDY DESIGN: We...
Topics
- Automation, Laboratory
- Drug Resistance, Viral
- Genotyping Techniques
- HIV Infections
- HIV-1
- High-Throughput Nucleotide Sequencing
- Humans
- Longitudinal Studies
- Mutation