Article
HIV-1 genotypic resistance testing using the Vela automated next-generation sequencing platform.
The Journal of antimicrobial chemotherapy - 1 May 2018
Raymond Stéphanie, Nicot Florence, Carcenac Romain, Lefebvre Caroline, Jeanne Nicolas, Saune Karine, Delobel Pierre, Izopet Jacques
Abstract excerpt
Objectives: To evaluate the diagnostic performance of the Vela next-generation sequencing (NGS) system in conjunction with the Sentosa SQ HIV Genotyping Assay for genotyping HIV-1. Methods: Plasma RNA was extracted and templates prepared with the Sentosa SX instrument before sequencing the HIV-1 polymerase on the Sentosa SQ301 Sequencer (PGM IonTorrent). The Vela NGS System was compared with direct sequencing and...
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