Article
Ultra-Sensitive Mutation Detection and Genome-Wide DNA Copy Number Reconstruction by Error-Corrected Circulating Tumor DNA Sequencing.
Clinical chemistry - 1 Nov 2018
Mansukhani Sonia, Barber Louise J, Kleftogiannis Dimitrios, Moorcraft Sing Yu, Davidson Michael, Woolston Andrew, Proszek Paula Zuzanna, Griffiths Beatrice, Fenwick Kerry, Herman Bram, Matthews Nik, O'Leary Ben, Hulkki Sanna, Gonzalez De Castro David, Patel Anisha, Wotherspoon Andrew, Okachi Aleruchi, Rana Isma, Begum Ruwaida, Davies Matthew N, Powles Thomas, von Loga Katharina, Hubank Michael, Turner Nick, Watkins David, Chau Ian, Cunningham David, Lise Stefano, Starling Naureen, Gerlinger Marco
Abstract excerpt
BACKGROUND: Circulating free DNA sequencing (cfDNA-Seq) can portray cancer genome landscapes, but highly sensitive and specific technologies are necessary to accurately detect mutations with often low variant frequencies. METHODS: We developed a customizable hybrid-capture cfDNA-Seq technology using off-the-shelf molecular barcodes and a novel duplex DNA molecule identification tool for enhanced error correction....
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.