Article
Error-corrected flow-based sequencing at whole-genome scale and its application to circulating cell-free DNA profiling.
Nature methods - 1 May 2025
Cheng Alexandre Pellan, Widman Adam J, Arora Anushri, Rusinek Itai, Sossin Aaron, Rajagopalan Srinivas, Midler Nicholas, Hooper William F, Murray Rebecca M, Halmos Daniel, Langanay Theophile, Chu Hoyin, Inghirami Giorgio, Potenski Catherine, Germer Soren, Marton Melissa, Manaa Dina, Helland Adrienne, Furatero Rob, McClintock Jaime, Winterkorn Lara, Steinsnyder Zoe, Wang Yohyoh, Alimohamed Asrar I, Malbari Murtaza S, Saxena Ashish, Callahan Margaret K, Frederick Dennie T, Spain Lavinia, Sigouros Michael, Manohar Jyothi, King Abigail, Wilkes David, Otilano John, Elemento Olivier, Mosquera Juan Miguel, Jaimovich Ariel, Lipson Doron, Turajlic Samra, Zody Michael C, Altorki Nasser K, Wolchok Jedd D, Postow Michael A, Robine Nicolas, Faltas Bishoy M, Boland Genevieve, Landau Dan A
Abstract excerpt
Differentiating sequencing errors from true variants is a central genomics challenge, calling for error suppression strategies that balance costs and sensitivity. For example, circulating cell-free DNA (ccfDNA) sequencing for cancer monitoring is limited by sparsity of circulating tumor DNA, abundance of genomic material in samples and preanalytical error rates. Whole-genome sequencing (WGS) can overcome the low...
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