Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Directed evolution of excited state lifetime and brightness in FusionRed using a microfluidic sorter.

Integrative biology : quantitative biosciences from nano to macro - 17 Sept 2018

Manna Premashis, Hung Sheng-Ting, Mukherjee Srijit, Friis Pia, Simpson David M, Lo Maria N, Palmer Amy E, Jimenez Ralph

Abstract excerpt

Green fluorescent proteins (GFP) and their blue, cyan and red counterparts offer unprecedented advantages as biological markers owing to their genetic encodability and straightforward expression in different organisms. Although significant advancements have been made towards engineering the key photo-physical properties of red fluorescent proteins (RFPs), they continue to perform sub-optimally relative to GFP...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cell Separation
Share to Directed Molecular Evolution

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Fluorescence

Share to Lab-On-A-Chip Devices

Share to Luminescent Proteins

Share to Microfluidics

Share to Protein Engineering

Share to Red Fluorescent Protein

Source

Open in PubMed Open full text

Identifiers

PMID: 30094420
DOI: 10.1039/c8ib00103k
PMCID: PMC6141309

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Directed evolution of excited state lifetime and brightness in FusionRed using a microfluidic sorter.PMID 30094420Read ↗

Fluorescence lifetime multiplexing with fluorogen activating protein FAST variants.Semantic proximity Read ↗

Bright GFP with subnanosecond fluorescence lifetime.Semantic proximity Read ↗

Improved Chemical-Genetic Fluorescent Markers for Live Cell Microscopy.Semantic proximity Read ↗

The first mutant of the Aequorea victoria green fluorescent protein that forms a red chromophore.Semantic proximity Read ↗

Influence of Fluorescence Lifetime Selections and Conformational Flexibility on Brightness of FusionRed Variants.Semantic proximity Read ↗

Functional tuning of a salvaged green fluorescent protein variant with a new sequence space by directed evolution.Semantic proximity Read ↗

Functional imaging-guided cell selection for evolving genetically encoded fluorescent indicators.Semantic proximity Read ↗

Molecular probes: insights into design and analysis from computational and physical chemistry.Semantic proximity Read ↗

Minimum set of mutations needed to optimize cyan fluorescent proteins for live cell imaging.Semantic proximity Read ↗

Analysis of DsRed Mutants. Space around the fluorophore accelerates fluorescence development.Semantic proximity Read ↗

Principles and applications of green fluorescent protein-based biosensors: a mini-review.Semantic proximity Read ↗

Recovery of red fluorescent protein chromophore maturation deficiency through rational design.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.