Article
Recovery of red fluorescent protein chromophore maturation deficiency through rational design.
PloS one - 1 Jan 2012
Moore Matthew M, Oteng-Pabi Samuel K, Pandelieva Antonia T, Mayo Stephen L, Chica Roberto A
Abstract excerpt
Red fluorescent proteins (RFPs) derived from organisms in the class Anthozoa have found widespread application as imaging tools in biological research. For most imaging experiments, RFPs that mature quickly to the red chromophore and produce little or no green chromophore are most useful. In this study, we used rational design to convert a yellow fluorescent mPlum mutant to a red-emitting RFP without reverting...
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