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Article

Functional tuning of a salvaged green fluorescent protein variant with a new sequence space by directed evolution.

Protein engineering - 1 Dec 2003

Nam Sung-Hun, Oh Ki-Hoon, Kim Geun-Joong, Kim Hak-Sung

Abstract excerpt

We previously reported a method, designated functional salvage screen (FSS), to generate protein lineages with new sequence spaces through the functional or structural salvage of a defective protein by employing green fluorescent protein (GFP) as a model protein. Here, in an attempt to mimic a step in the natural evolution process of proteins, the functionally salvaged mutant GFP-I5 with new sequence space, but...

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PMID: 14983092
DOI: 10.1093/protein/gzg146

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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