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Article

Fluorescence lifetime multiplexing with fluorogen activating protein FAST variants.

Communications biology - 2 Jul 2024

Bogdanova Yulia A, Solovyev Ilya D, Baleeva Nadezhda S, Myasnyanko Ivan N, Gorshkova Anastasia A, Gorbachev Dmitriy A, Gilvanov Aidar R, Goncharuk Sergey A, Goncharuk Marina V, Mineev Konstantin S, Arseniev Alexander S, Bogdanov Alexey M, Savitsky Alexander P, Baranov Mikhail S

Abstract excerpt

In this paper, we propose a fluorescence-lifetime imaging microscopy (FLIM) multiplexing system based on the fluorogen-activating protein FAST. This genetically encoded fluorescent labeling platform employs FAST mutants that activate the same fluorogen but provide different fluorescence lifetimes for each specific protein-dye pair. All the proposed probes with varying lifetimes possess nearly identical and the...

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Identifiers

PMID: 38956304
DOI: 10.1038/s42003-024-06501-1
PMCID: PMC11219735

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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