Article
N-glycosylation in the protease domain of trypsin-like serine proteases mediates calnexin-assisted protein folding.
eLife - 11 Jun 2018
Wang Hao, Li Shuo, Wang Juejin, Chen Shenghan, Sun Xue-Long, Wu Qingyu
Abstract excerpt
Trypsin-like serine proteases are essential in physiological processes. Studies have shown that N-glycans are important for serine protease expression and secretion, but the underlying mechanisms are poorly understood. Here, we report a common mechanism of N-glycosylation in the protease domains of corin, enteropeptidase and prothrombin in calnexin-mediated glycoprotein folding and extracellular expression. This...
Topics
- Animals
- Binding Sites
- Calnexin
- Cell Line
- Glycosylation
- HEK293 Cells
- Hep G2 Cells
- Humans
- Mutation
- Phylogeny
- Polysaccharides
- Protein Domains
- Protein Folding
- Serine Endopeptidases